Click here for table of content Catalogue BioScience Edition 04 / 2018 www.gbo.com

Product Highlights CELLdiscTM – Innovative Multi-Layer Device for Mass Cell Culture • Ergonomic round system for the propagation of adherent cells • 40 % higher surface / volume ratio than competitive systems • CELLSTAR® TC and Advanced TC™ surfaces assure high cell yield • Centrally located gas exchange channel for uniform distribution Magnetic 3D Cell Culture • Rapid development of three-dimensional model structures as well as co-culture of various cell lines • Easy-to-use in different formats (from single well to 384 well format) • No sample loss during experimental runs One click to our products  p. 41 – 42  p. 60 – 65 CELLview™ Plates – Glass Bottom Microplates for High Resolution Microscopy • Cycloolefin-based black frame with 0.17 mm glass bottom for superior imaging • Recessed bottom geometry facilitates imaging of all peripheral wells • Appropriate surface treatment for improved cellular attachment and growth  p. 70 Sapphire PCR Consumables • Thin-walled polypropylene for efficient heat transfer during amplification • Free of detectable DNase, RNase, human DNA, endotoxin and PCR inhibitors • Suitable for real-time / quantitative PCR Triple-Packed Products for Cleanroom Applications • Convenient and easy clean room handling • No need for alcohol wipe downs or cleaning processes • High sterilisation level (SAL 10 – 6) for maximum security • Three individual easy-to-open peel bags  p. 176 – 182 TRI  p. 200 – 205 Barcoded Cryo.sTM for Biobanking & Accessories • Cryo.s™ with Datamatrix code, linear barcode and human readable ID on each tube • Cryo.s™ Biobanking Tubes for space-efficient sample storage, best in class Datamatrix code • Cryo.s™ Rack Scanner for the reliable and fast identification of tube and rack IDs • Cryo.s™ 8-Channel Handheld Decapper for efficient tube closure  p. 213 – 219 Lab Equipment for Greiner Bio-One Consumables • 6 different small lab equipment devices • Mini versions with compact design fit in the palm of your hand • General 2 year “no hassle” warranty  p. 236 – 240 e u s s T / i l l e C 1 e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience i l a c n h c e T 5 1 i x d n e p p A

Greiner Bio-One – Your Power for Health Over seven billion people inhabit the earth today. As diverse as they all may be, one desire unites everybody: The desire for a healthy, vital life. We know that we can make a significant contribution to the areas of health and safety in medicine. At the same time, this knowledge means a great responsibility, which we face up to with a high level of personal commitment and innovative solutions. Products from Greiner Bio-One are applied worldwide from pure research to drug discovery to preanalytics or diagnostics. Thus we can offer scientists in many countries suitable platforms for their research work, laboratories the equipment for precise analysis and health professionals a basis for reliable diagnosis and therapy. Offering an unsurpassed scope of products and services across a variety of disciplines, we focus on the evolving needs of our customers in the development of innovative products, to refine existing technologies, and redefine the state of the art. Our fast and flexible response in customer-specific projects, combined with multiple strategic manufacturing sites, ensures an uninterrupted supply of critical components to meet your product needs. With worldwide locations and effective global distribution, you will always be within reach of the product, service and support you need. Greiner Bio-One has many international production sites and distribution centres: Germany • Austria • Belgium • Brazil • China France • Hungary • India • Italy • Japan • Netherlands Portugal • Spain • Thailand • United Kingdom • USA More than 2000 employees throughout the world are dedicated to the aim of constantly improving the life quality of all people. We put the Power in Health Protection. e e u u s s s s T T / / i i l l l l e e C C 1 1 - - S S T T H H 2 2 l l / / y y g g o o o o n n u u m m m m I I 3 3 i i l l / / y y g g o o o o b b o o r r c c M M 4 4 i i s s r r e e k k a a e e B B / / s s e e b b u u T T 5 5 g g n n i i l l d d n n a a H H d d u u q q L L i i i i 6 6 r r a a l l l l u u c c e e o o M M 7 7 n n i i e e t t o o r r P P 8 8 n n o o i i t t a a r r a a p p e e S S 9 9 d d e e k k c c a a P P - - e e p p i i r r T T 0 0 1 1 l l i i s s c c n n h h c c e e t t o o y y r r C C 1 1 1 1 l l / / s s r r e e a a e e S S / / s s d d L L 2 2 1 1 i i / / s s e e b b u u T T n n o o i i t t c c a a e e R R 3 3 1 1 s s e e i i r r o o s s s s e e c c c c A A 4 4 1 1 i i l l a a c c n n h h c c e e T T 5 5 1 1 www.gbo.com/bioscience 5 www.gbo.com/bioscience 5 e e r r u u t t l l u u C C l l s s e e t a t a p p o o r r c c M M i i A A L L H H l l y y g g o o o o i i r r e e t t c c a a B B y y g g o o o o B B l l i i n n o o i i t t a a s s i i l l l l a a t t s s y y r r C C s s t t c c u u d d o o r r P P s s t t a a M M p p a a C C s s p p u u C C r r e e s s y y a a n n A A l l i i x x d d n n e e p p p p A A

e u s s T / i l l e C 1 e r u t l u C Quality Control System Control of incoming goods Incoming goods used for production (e. g. raw materials, foils, etc.) are checked for purity, identi- ty and if they are in accordance with the agreed specifications. In-process control (IPC) During the manufacturing process, the quality of the products is checked periodically based on individual testing plans according to the specifications. Quality control in the laboratory Specific products are tested for physical, chemical and biological functionality (see below). Final control In addition to dimensional, visual and functional criteria, products are finally tested for labelling, packaging and documentation. - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i l s e t a p o r c M i A L H l y g o o i r e t c a B Products from Greiner Bio-One provide one or more of the following application-dependent features: Free of detectable DNase, RNase and human DNA For reliable and accurate results in molecular biology applications, e. g. sequencing and amplification, the applied products are free of detectable DNase, RNase and human DNA. Therefore protec- tive. g.ar, like overalls, gloves and hairnets, is a prerequisite in all production areas. Furthermore, a routine monitoring of DNase, RNase and human DNA is performed. Products are washed with an appropriate volume of a 0.5 % Tween 20 solution to detach adherent nucleases and nucleotides. DNase, RNase and human DNA present in the washing solution are detected by Real Time PCR. The detection limits*) related to the applied PCR methods are 1.3 × 10 -8 Kunitz units for DNase, 5 × 10-11 Kunitz units for RNase and 5 pg for human DNA. All products that meet the above mentioned features are identified in our catalogue with the respective label. Free of detectable endotoxins Endotoxins are complex lipopolysaccharides and part of the outer membrane of gram-negative bacteria. These substances are the most widespread and effective species of a group of so- called pyrogens that can cause fever. The US Pharmacopoeia guidelines stipulate that eluates from Medical Devices (e. g. washing fluids from disposables) do not exceed endotoxin levels of more than 0.5 EU / ml. For consumables that are in contact with cerebrospinal fluid, the endotoxin level should be at a minimum value of 0.06 EU / ml. The testing of endotoxins is conducted in a kinetic turbidimetric LAL assay (Limulus Amoebocyte Lysate) with a detection limit of 0.03 EU / ml*). This test procedure complies with the United States Pharmacopoeia (USP) Chapter <85> Bacterial Endotoxins Test. All products that meet the above mentioned feature are identified in our catalogue with the respective label. Sterility The validation of our products is conducted in accordance with ISO 11137. Related to this standard, the bioburden (germ level) is established for each product and periodically repeated. Based on this data the necessary sterilisation process is conducted. Products labelled as sterile are routinely monitored. In-Vitro Diagnostic and Medical Devices appear with an SAL (Sterility Assurance Level) of 10-6, other sterile products meet an SAL of 10-3. Binding properties of immunological products The binding properties of all our immunological products are tested based on ELISA. The limits of the coefficient of variation (CV) of our products are as follows: MICROLON® CV < 5 %; FLUOTRACTM and LUMITRACTM CV < 10 %. We provide these products with a shelf life of 4 years for the binding characteristics. Cell compatibility of cell culture products The quality of cell culture products is validated with different cell lines. Relevant parameters like morphology, cell growth and cell vitality are monitored. More detailed information about the shelf life of our cell culture products is listed in the product data sheets on our website. Free of cytotoxic substances Cell culture products used in cell-based applications and for In-Vitro Diagnostic Devices should not contain any cytotoxic substances. Therefore all relevant cell culture end products are analysed for cytotoxicity. The detection of cytotoxicity is evaluated with mammalian cells according to EN ISO 10993-5. All products that meet the above mentioned feature are identified in our catalogue with the respective label. *) More information can be found on our website www.gbo.com. Errors and omissions excepted. s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 7 i l a c n h c e T 5 1 i x d n e p p A

Download Center Your gateway to a wealth of information The Download Center has been developed to house our complete library of information about Greiner Bio-One products and to make it easier to find and access this information. The download area contains instructions for use, product brochures, product data sheets, surveys and much more. If you are looking for a specific document, then the search function offers you various ways to search for it. You can search by product number, product category or by different language versions of documents. Frequently Asked Questions (FAQs) You Ask – We Answer Our FAQ section offers you the ability to send your question about Greiner Bio-One products and services straight to us. Our product specialists will be happy to assist you with your query and to make your daily work with Greiner Bio-One products even easier. Search for already existing FAQs using the free text search function or browse through the product categories for further information. The FAQs section is constantly updated with your questions and answers from Greiner Bio-One product experts. E-Mail Newsletter Stay up-to-date Our e-mail newsletter is one of our most popular methods of keeping our customers informed. Subscribe today and stay updated with news around Greiner Bio-One. The newsletter will be sent out 2 to 3 times a year. Our Newsletter keeps you updated about the latest product developments, new services, publications and much more. Additionally we send out invitations for Greiner Bio-One webinars, fairs and congresses. e u s s T / i l l e C 1 e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 9 i l a c n h c e T 5 1 i x d n e p p A

Production Greiner Bio-One, a technology leader in manufacturing high quality plastic products, relies on cutting-edge production facilities. These installations are powered with clean, renewable forms of energy. Fossil fuels such as gas or fuel oil are not being used. Energy is supplied in the form of electric power only. The sprue parts, for example, that accumulate in every injection moulding process are recycled. After a homogenous selection of the remaining production wastes, they are delivered to a compounding contractor that recycles the material into plastic granulate for reuse in the injection moulding industry. Most plastic materials permit the recovery of either materials or energy. Based on generally ecological considerations, the recovery of materials is preferable, provided they can be used in the place of new plastic materials. It is for this reason that a special recycling solution helps the machinery save on materials. A state-of-the-art heat recovery system utilises the waste heat generated by the production machines to heat the entire building complex and help save additional energy. Packaging Wherever possible, unnecessary packaging material is dispensed with. The cartons consist of 100 % recycled material. All cartons from Greiner Bio-One Frickenhausen are labelled with the RESY symbol. This means that the RESY Organisation für Wertstoffentsorgung GmbH guarantees the complete disposal and reuse of all transport and grouped packaging bearing this symbol. Energy Management System The Greiner Bio-One site in Frickenhausen began in troducing an energy management system as early as 2012 and has been certified to DIN EN ISO 50001 since 2013. All energy flows were analysed and valued so that steps could then be taken to optimise energy efficiency. Improving the energy balance while also reducing CO2 emissions was a huge incentive for the management team and workforce alike. As a result, the company succeeded in saving 650,000 kWh of electricity in 2016 alone. The company has also been recertified to DIN EN ISO 50001 in 2016 successfully. Introducing an energy management system is funda- mentally a voluntary undertaking, as there is still no legal requirement to obtain certification. Greiner Bio-One nonetheless long ago established its own energy team that identifies potential energy savings. In addition, the team trains and motivates the workforce to achieve the shared goals of cutting energy costs and thus contribut- ing to halting climate change. DIN EN ISO 50001 Certification e u s s T / i l l e C 1 e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 11 i l a c n h c e T 5 1 i x d n e p p A

OEM Service Portfolio Greiner Bio-One manufactures numerous products through injection moulding and offers the entire product development and production process (Fig. 2) from an idea to the finished product: From design, via prototyping, through fully automated manufacturing, Greiner Bio-One delivers the complete solution with support of experienced scientists, engineers and specialists. Research & Development with diverse application and research laboratories Construction of moulds, planning of plants and assembly lines Modern, fully automated production Post production according to customers’ requirements Comprehensive quality assurance and management system Worldwide networked warehousing and customer service GLOBAL SERVICE RESEARCH & DEVELOPMENT CONSTRUCTION QUALITY PRODUCTION Figure 2: OEM service portfolio POST PRODUCTION Always close to the customer Always highest quality standards As a global player and single source supplier, Greiner Bio-One offers ultra-modern production plants on 4 continents – with a centralised quality management system and a global procurement policy. As a result, customer-oriented production activities, high capacity, global warehousing and rapid delivery can be ensured. A code of conduct also governs worldwide company and employer business practices. With a global sales network specifically established for the field of OEM, Greiner Bio-One offers custom-made solutions with personal service. Quality is critical at Greiner Bio-One. All production sites are certified according to the international standards DIN EN ISO 9001, EN ISO 13485 as well as complying with FDA and CAMDCAS requirements for producers of medical products. Greiner Bio-One has established a multistage quality assurance system across all production sites. This starts with the goods-in control for raw materials. Further legal and customer-specified controls are applied throughout production. In addition, products are tested for compliance with customer-specific qualities such as sterility. Products are only considered as finished following strict quality checks. This is our quality promise. e u s s T / i l l e C 1 e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 13 i l a c n h c e T 5 1 i x d n e p p A

One click to each chapter 1 Cell and Tissue Culture 2 HTS-Microplates 3 Immunology / HLA 4 Microbiology / Bacteriology 5 Tubes / Beakers 6 Liquid Handling 7 Molecular Biology 8 Protein Crystallisation 9 Separation 10 Triple-Packed Products TRI 11 Cryotechnics 12 Lids / Sealers / CapMats 13 Reaction Tubes / Analyser Cups 14 Accessories We reserve the right to make changes, deletions or additions. We cannot accept any responsibility for typographical errors or printing omissions. e u s s T / i l l e C 1 e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 15 Technical Appendix www.gbo.com/bioscience 15 i l a c n h c e T 5 1 i x d n e p p A

1 Cell and Tissue Culture Technical Information Cell Culture Products CELLSTAR® Cell Culture Flasks – Standard Cell Culture Flasks – Filter Cap Cell Culture Flasks – Standard Suspension Culture Flasks – Filter Cap Suspension Culture Flasks – AutoFlaskTM CELLSTAR® Cell Culture Dishes CELLSTAR® OneWell PlateTM CELLSTAR® FourWell PlateTM CELLSTAR® Cell Culture Multiwell Plates CELLSTAR® Cell Culture Microplates – 96 Well Cell Culture Microplates – 384 Well Cell Culture Microplates – 1536 Well Cell Culture Microplates CELLSTAR® Cell Culture Tubes CELLSTAR® CELLreactorTM EASYstrainerTM Cell Strainers Mass Cell Culture CELLMASTERTM Cell Culture Roller Bottles CELLdiscTM CELLswingTM Advanced TCTM Cell Culture Vessels CELLCOAT® Protein Coated Cell Culture Vessels 3D Cell Culture CELLSTAR® Cell Culture Vessels with Cell-Repellent Surface Magnetic 3D Cell Culture Cell Culture Products for Microscopy CELLviewTM Dish CELLviewTM Slide CELLviewTM Plate SCREENSTAR Microplates Accessories Cell Scrapers ThinCertTM ThinCertTM Cell Culture Inserts ThinCertTM Cell Culture Inserts coated with Collagen Type I ThinCertTM Plate 18 20 20 20 21 22 22 23 24 25 25 26 27 27 31 33 34 35 36 37 37 41 43 44 48 56 57 60 66 68 69 70 71 73 73 74 74 78 79 e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 17 i l a c n h c e T 5 1 i x d n e p p A

3. Surface Treatment The surfaces of CELLSTAR® and CELLMASTERTM products for adherent cell culture are treated using a special physical method. This treatment leads to polar groups, such as carboxy and hydroxy groups, being incorporated into the plastic surface, making it hydrophilic. This significantly improves the adhesion of cells and the binding of proteins to the plastic surface. Cell culture treated products are labelled with TC surface treatment (TC = Tissue Culture) in the catalogue. For the cultivation of fastidious cells or cells cultivated under restricted growth conditions Greiner Bio-One offers the Advanced TC™ polymer modification. Based on this innovative technique, the cell culture surface is modified in such a way as to positively influence cellular features and functions. Enhanced cell attachment and higher pro- liferation rates improve and accelerate cell expansion. In addition to the physically and chemically modified surfaces for cell culture, we also provide products coated with Collagen Type I, Poly-D-Lysine, Poly-L-Lysine, Fibronectin and Laminin. These coatings facilitate the growth of many cell types, including hepatocytes, muscle cells, epithelial/endothelial cells, neural cells and transfected cell lines. The products are summarised in our CELLCOAT® product range. Our suspension cell culture vessels with their strongly hydrophobic surfaces are particularly well suited for non-adherent cell cultures, hybridomas and embryonic stem cell cultures. CELLSTAR® cell culture vessels with a cell-repellent surface reliably prevent cell attachment in suspension cultures of semi-adherent and adherent cell lines where standard hydrophobic surfaces generally used for suspension culture are insufficient. 4. Screw Caps with a Hydrophobic Capillary Pore Membrane Filter screw caps for cell culture / suspension culture flasks, roller bottles and the CELLreactorTM have a patented hydrophobic capillary pore membrane (Fig. 1). The defined and constant pore size of 0.2 µm is achieved with minimal variation by means of a specially developed, high- technology method (Fig. 2). Figure 2: Comparison of different membrane types according to their pore size distribution The filter insert provides both optimal protection against contamination and efficient gas exchange. By using PET / PTFE which are responsible for the mechanical strength and hydrophobic properties of the membrane, these advantages are retained even if the inside of the cap is briefly wetted with medium. 5. Expiry Date / Lot Number All cell culture products are labelled with expiry date and lot number in order to ensure transparency of product processes and retraceability of our products throughout the production process. 6. Quality Control Based on automated production processes with quality controls, we provide immaculate, high-quality products for all areas of cell culture. All CELLSTAR® and CELLMASTERTM products as well as ThinCertTM, Advanced TCTM and CELLviewTM are sterilised by irradiation. They are controlled for sterility as well as for absence of detectable endotoxins, DNase / RNase and human DNA ( Quality p. 6 – 7). Cytotoxicity In many experiments toxic effects on cells or tissue cultures play an important role particularly in the field of In-Vitro Diagnostics. The absence of any adverse biological reaction due to contact with our production materials, e.g. polystyrene or polypropylene, is closely monitored by cultivating cells in the presence of extracts from these polymers. The detection of cytotoxicity is evaluated with mammalian cells according to EN ISO 10993 – 5. All cell culture relevant end products are analysed for the absence of cytotoxic components. Therefore the end product is cultivated with cell culture media. The generated extract is then added to a sensitive cell culture for 24 hours. a) b) Further information on cell culture products Figure 1: a) Top view of the capillary pore membrane (electron micrograph) b) Cross-section of the capillary pore membrane: the capillaries are filled with copper and the PET is then removed by etching (electron micrograph)  Application Note “siRNA-dependent gene silencing on various cell culture surfaces” (F071105)  Application Note “Improved cultivation / differentiation of embryonic stem cells” (F073117)  Application Note “Cultivation and differentiation of hADSCs with CELLSTAR® and CELLCOAT® products” (F073113) / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 19 i l a c n h c e T 5 1 i x d n e p p A e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i s t c u d o r P s t a M p a C s p u C r e s y a n A l

CELLSTAR® Cell Culture Products CELLSTAR® Filter Cap Cell Culture Flasks 690175 Filter Cap Cell Culture Flasks 658175 660175 661175 A specific capillary pore membrane is used for the filter screw caps of our filter cap cell culture flasks. The defined pore diameter of 0.2 µm provides a sterile barrier against contamination. The inner surface of the PET membrane is PTFE-coated generating a hydrophobic facing which prevents wetting of the filter material from internal liquid. Due to the high airflow rate of the filter material, an optimal gas exchange is ensured. Cat.-No. 690175 / 658175 / 660175 also available triple-packed p. 204 TRI • Improved cell adhesion • Sterile and user-friendly through physical surface treatment packaging • Cell culture flasks with filter screw cap • Canted neck • Graduation on both sides • 25 cm2, 75 cm2 and 175 cm2 growth areas • High and flat design of the 175 cm2 cell culture flasks for variability of media volume • High airflow rate and optimal gas exchange • Additional standard screw caps (without filter) available on request Cat.-No. Flask design Growth area [cm2] Total volume [ml] Working volume [ml] TC surface treatment Sterile Filter screw cap 690175 658175 660175 661175 - 25 50 - 75 250 flat 175 550 high 175 650 5 – 10 15 – 38 20 – 45 20 – 85 Quantity per bag / case 10 / 200 5 / 120 + + red + + red + + red 5 / 50 + + red 4 / 40 e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B i l n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 21 i l a c n h c e T 5 1 i x d n e p p A

CELLSTAR® AutoFlaskTM CELLSTAR® Cell Culture Products 779190 AutoFlaskTM • Compatible with a wide range of cell culture and liquid handling systems • Hydrophobic membrane for optimal gas exchange • Format Length: 127.76 mm Width: 85.48 mm Height: 19.5 mm • Pre-scored multiple entry septum • Centrifugation pocket for cell separation • Customisable barcode labelling • Handling and pipetting in horizontal position • Different coatings and surface modifications available 779160 AutoFlaskTM – Cell culture flask for automated systems The standard microplate footprint of the AutoFlaskTM ensures compatibility with a wide range of cell culture and liquid handling systems. A robotically accessible pre-scored multiple entry septum (Fig. 1  1) assures sterility of flask contents throughout processing. The unique centrifugation pocket (Fig. 1  2) enables separation of cells from supernatant inside the flask and the integrated hydrophobic filter (Fig. 1  3) facilitates gas exchange during the cultivation of cells. A user-friendly colour coding (Fig. 1  4) allows easy identification of the AutoFlaskTM version. Beside the cell culture treated and the suspension culture version, Greiner Bio-One also offers the AutoFlaskTM with Poly-D-Lysine ( p. 51) or Collagen Type I ( p. 49) coating as well as with Advanced TCTM polymer modification ( p. 45). 3 1 2 4 Further information on the AutoFlaskTM Figure 1: CELLSTAR® AutoFlaskTM  “Comparative cell growth study using the AutoFlaskTM” (F072094) e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 Cat.-No. Description TC surface treatment Sterile Growth area [cm2] Total volume [ml] Working volume [ml] Colour code Barcode labelling Quantity per bag / case d e k c a P - e p i r T 0 1 l 779160 779190 AutoFlaskTM AutoFlaskTM i s c n h c e t o y r C 1 1 + + 83.6 110 20 – 40 red + -/suspension + 83.6 110 60 – 80 white + 10 / 100 10 / 100 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 s t c u d o r P s t a M p a C s p u C r e s y a n A l www.gbo.com/bioscience 23 i l a c n h c e T 5 1 i x d n e p p A

CELLSTAR® Cell Culture Products CELLSTAR® OneWell PlateTM and FourWell PlateTM CELLSTAR® OneWell PlateTM Non-divided plate for tissue culture applications CELLSTAR® FourWell PlateTM Subdivided plate for microscopic applications The CELLSTAR® OneWell PlateTM can be used if large quantities of cells have to be cultivated. The external dimensions comply with ANSI standards to render the plate suitable for use on a wide range of cell culture and liquid handling systems. With a growth area of 95 cm2, the OneWell PlateTM fills the gap between the growth areas of 58 cm2 (Cat.-No. 664160) and 143 cm2 (Cat.-No. 639160) in the cell culture dish product range. Handling and the required incubator space are improved compared to a round cell culture dish. Notches on the left side of the plate and the lid ensure a secured lid position. The TC-treated version is provided with a proprietary physical surface treatment increasing the hydrophilicity of the plate and facilitating the cultivation of adherent cells. As with all Greiner Bio-One CELLSTAR® products, the CELLSTAR® OneWell PlateTM is made of high grade polystyrene and is guaranteed to be sterile, non-pyrogenic, non-cytotoxic and free of detectable DNase, RNase and human DNA. Beside general tissue culture applications the CELLSTAR® OneWell PlateTM can be used as a multipurpose liquid container or disposable for the denaturation, hybridisation and washing of membranes (Southern, Northern and Western Blot). The CELLSTAR® FourWell PlateTM facilitates the cultivation of cells and the storage of microscopic slides in an HTS- compatible plate complying with the ANSI standard. With its four subdivisions the plate offers space for four individual slides with standard dimensions and enables four parallel experiments. Cells and tissue samples cultivated on these slides can be supplied quickly with fresh media and can be examined directly under a microscope. Thereafter, samples can also be fixed and analysed by immunohisto- and immunocytochemical techniques. Notches on the left side and a numbering of each individual compartment make a laterally reversed usage or confusion of samples impossible. A semicircular recession at the top and at the bottom of each compartment enables easy removal and handling of slides. The two pins at the left and right side of the semicircular recession hinder the microscopic slide to adjoin the outer rim of the plate and to fully cover the recession. This guarantees that the slide can always be removed manually from the compartment even if it adheres to the plate bottom due to capillary forces. Beyond the indicated microscopic applications, the CELLSTAR® FourWell PlateTM can also be used as a liquid container or disposable for the denaturation, hybridisation and washing of membranes (Southern, Northern and Western Blot). OneWell PlateTM FourWell PlateTM CELLSTAR® OneWell PlateTM (non-sterile) for bacteriological applications p. 134 96077307 670180 670190 Cat.-No. Description No. of chambers Length [mm] x width [mm] Height [mm] Total volume [ml] Growth area [cm2] Notches TC surface treatment Sterile Lid Quantity per bag / case 670180 670190 96077307 OneWell PlateTM OneWell PlateTM FourWell PlateTM 1 1 4 127.8 x 85.5 127.8 x 85.5 127.8 x 85.5 14.4 113.7 95 + + + + 14.4 113.7 95 + - + + 14.4 18.6 / well - + - + + 8 / 32 8 / 32 8 / 32 e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 25 i l a c n h c e T 5 1 i x d n e p p A

CELLSTAR® Cell Culture Microplates Cell culture treated microplates are available in the following versions: 96, 384, 1536 well format Properties Improved cell adhesion through physical surface treatment Compatible with automated systems Alphanumeric well coding An overview of all 96 well, 384 well and 1536 well microplates listed in this catalogue can be found in the Technical Appendix  p. 245 – 247 CELLSTAR® Cell Culture Products Detailed technical information on microplates  p. 82 – 85 HTS microplates  p. 120 – 121 Immunology  p. 243 Barcode labelling of microplates Further literature on cell culture treated microplates  Application Note “Establishing a cell culture assay based on TR-FRET for screening G-Protein-coupled receptors” (F074058)  Application Note “Selection of cell culture surfaces for the adipogenic differentiation of hMSCs” (F010003) 96 Well Polystyrene Cell Culture Microplates Cell culture treated 96 well microplates are available in the following versions: With U-, V- and F-bottom Clear, black and white Standard or half area microplates Black and white “clear bottom” plates (µClear®) Chimney well design, raised wells and condensation rings in lids prevent cross-contamination With or without lid Improved cell adhesion through physical surface treatment Barcode-labelled on request Properties Lid enables gas exchange with minimal evaporation High clarity of the clear microplates for optimal microscopic examinations Stackable Alphanumeric well coding Individually wrapped peel-off bags Consecutive lot numbering Well Profile Depending on the application, the well profile is a key feature in a 96 well cell culture microplate. For further information and figures on the well profiles see  p. 86 – 87. 4. F-Bottom / Chimney Well The chimney well cell culture microplate has the same well profile as the standard F-bottom plate. The difference to the standard plate is the chimney-like arrangement of the wells i.e. each well stands on its own. Therefore the risk of conta- mination from sample material being carried over is minimised. Cell growth area: 34 mm2 Working volume: 25 – 340 µl µClear® / Solid Bottom Clear bottom microplates have pigmented walls and a transparent thin film bottom, the so-called µClear® bottom. In contrast to our standard microplates with a solid polystyrene bottom, they are ideal for cell culture and microscopic applications using fluorescence or luminescence detection methods. Half Area Microplates For many applications, a reduction of the sample volume is an important feature. Beside high-format plates, the 96 well half area microplates offer an interesting alternative here. They can be pipetted automatically as well as manually without any pro- blem and allow a reduction of the sample volume up to 50 %. Cell growth area: 15.0 mm2 Working volume: 15 – 175 µl e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 d e k c a P - e p i r T 0 1 l s t c u d o r P s t a M p a C s p u C r e s y a n A l 1. U-Bottom The “U” describes the round bottom shape. No sharp corners to facilitate easy and residue-free pipetting Suitable for +/- analyses Working volume: 40 – 280 µl 2. V-Bottom The “V” stands for the conically tapered well bottom. For precise pipetting Suitable for +/- analyses Working volume: 40 – 200 µl 3. F-Bottom / Standard (ST) The “F” refers to the flat well bottom. Excellent optical properties For precise optical measurements For microscopic applications (bottom reading) Cell growth area: 32 mm2 Working volume: 25 – 340 µl Bulk Packaging For selected products Greiner Bio-One also offers user-friendly bulk packaging. Additional products are available in bulk pack upon request. i s c n h c e t o y r C 1 1 New: Clear 96 well microplates (F-bottom / chimney well) now with black printing: • Designated surface treatment for improved • Batch number and expiry date for reliable • Alphanumeric code for identification traceability rapid orientation • Additional frosted labelling field on plate side l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 27 i l a c n h c e T 5 1 i x d n e p p A

CELLSTAR® Cell Culture Products 96 Well Polystyrene Cell Culture Microplates solid bottom, white / black 655079 655086 96 Well Microplates p. 88 Sealers, Lids and CapMats p. 222 – 227 655073 655083 Microplate Centrifuge p. 237 Barcode Labelling p. 243 Cat.-No. Well profile Bottom Colour Growth area per well [mm2] Working volume per well [µl] TC surface treatment Sterile Lid 655073 655083 655079 655086 F-bottom / F-bottom / F-bottom / F-bottom / chimney well chimney well chimney well chimney well solid white 34 solid white 34 solid black 34 solid black 34 25 – 340 25 – 340 25 – 340 25 – 340 + + - + + +*) + + - + + +*) Quantity per bag / case 10 / 40 8 / 32 10 / 40 8 / 32 *) with condensation rings e u s s T / l l i e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 www.gbo.com/bioscience 29 i l a c n h c e T 5 1 i x d n e p p A

CELLSTAR® Cell Culture Products e u s s T / l l i 384 Well Polystyrene Cell Culture Microplates 384 well cell culture microplates are physically surface treated for improved cell adhesion and available in the following versions: Clear, white or black colour White or black “clear bottom” plates (µClear®) 384 well Small VolumeTM HiBase microplates Barcode-labelled on request ( p. 243) e C 1 - S T H 2 l / y g o o n u m m I 3 l i / y g o o b o r c M 4 i e r u t l u C l s e t a p o r c M i A L H l y g o o i r e t c a B s r e k a e B / s e b u T 5 g n i l d n a H d u q L i i 6 r a l l u c e o M 7 y g o o B l i n o i t a s i l l a t s y r C n i e t o r P 8 n o i t a r a p e S 9 s t c u d o r P s t a M p a C s p u C r e s y a n A l d e k c a P - e p i r T 0 1 l i s c n h c e t o y r C 1 1 l / s r e a e S / s d L 2 1 i / s e b u T n o i t c a e R 3 1 s e i r o s s e c c A 4 1 781079 781086 384 Well Polystyrene Cell Culture Microplates solid bottom, clear / white / black 384 Well Microplates p. 93 Sealers, Lids and CapMats p. 222 – 227 Barcode Labelling p. 243 781073 781080 Properties: • Lid enables gas exchange with the lowest possible evaporation • High clarity of the clear microplates for optimal microscopic • Stackable • Alphanumeric well coding examinations Cat.-No. Well profile Bottom Colour Growth area per well [mm2] 781165 781182 781073 781080 781079 781086 F-bottom F-bottom F-bottom F-bottom F-bottom F-bottom solid clear 10 solid clear 10 solid white 10 solid white 10 solid black 10 solid black 10 Working volume per well [µl] 15 – 110 15 – 110 15 – 110 15 – 110 15 – 110 15 – 110 TC surface treatment Sterile Lid + + - + + + + + - + + + + + - + + + Quantity per bag / case 10 / 40 8 / 32 10 / 40 8 / 32 10 / 40 8 / 32 www.gbo.com/bioscience 31 i l a c n h c e T 5 1 i x d n e p p A